Plasmid

Part:BBa_K4427012:Design

Designed by: Chanjuan Jin   Group: iGEM22_JLU_China   (2022-09-30)


pSB1C3-stuffer-all


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 7094
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal PstI site found at 2400
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 7095
    Illegal PstI site found at 2400
    Illegal PstI site found at 7109
    Illegal NotI site found at 7
    Illegal NotI site found at 7102
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 1165
    Illegal BamHI site found at 7017
    Illegal XhoI site found at 1743
    Illegal XhoI site found at 7026
    Illegal XhoI site found at 8126
    Illegal XhoI site found at 9018
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 7095
    Illegal PstI site found at 2400
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 7095
    Illegal PstI site found at 2400
    Illegal PstI site found at 7109
    Illegal NgoMIV site found at 1313
    Illegal AgeI site found at 1000
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

e


Source

yea

References